Abstract: Objective The study aimed to detect and analyze the main biological characteristics of domestic commercial immunodeficient rats, including the percentage of blood lymphocyte subsets, histology of immune organs, organ coefficients, and blood physiological indexes. Method Six strains of immunodeficient rats of three categories commercially available in China were selected, as well as corresponding control with the same genetic background. The blood physiological indexes, blood lymphocyte subsets, organ coefficients of major immune organs, and histological features were detected and compared. Result ( 1) The thymus coefficient and histological features of T lymphocyte-deficient rats showed significant differences compared to the control group (P<0. 01) . The levels of WBC (white blood cells) , LYMPH ( lymphocytes) , LYMPH% ( lymphocyte percentage) , as well as the proportions of T cell, were significantly lower than those in the control group ( P<0. 01) . In contrast, the levels of NEUT ( neutrophils) , NEUT% ( neutrophil percentage) , the proportions of B cell, and the proportions of NK ( natural killer) cell were higher than those in the control group ( P<0. 01) . Significant differences (P<0. 01) were also observed in the spleen coefficient, WBC, LYMPH, LYMPH%, NEUT%, and the proportions of B cell among immunodeficient rats from different manufacturers. (2) The spleen coefficient and thymus coefficient of rats with combined T and B lymphocyte deficiency, as well as their histological features, showed significant differences compared to the control group ( P<0. 01) . The levels of WBC, LYMPH, NEUT %, the proportions of T cell, and the proportions of B cell were all significantly lower than those in the normal control group (P<0. 01) . In contrast, the percentages of MONO (monocytes) , NEUT, NEUT %, and the proportions of NK cell were significantly higher than those in the control group (P<0. 01) . (3) The spleen coefficient and thymus coefficient of rats with combined T, B, and NK cell deficiency were significantly lower than those of the control group ( P < 0. 05,P < 0. 01) . The levels of WBC, LYMPH, LYMPH % as well as the proportions of T cell and B cell, were all significantly lower than those of the control group (P<0. 01) . The percentages of NEUT and MONO were higher than those of the control group (P<0. 01) . Significant differences were observed in the LYMPH %, NEUT%, and MONO% among rats with combined T, B, and NK cell deficiency from different manufacturers ( P < 0. 01) . Conclusion The immune cell counts, histology of immune organs, and hematology indexes of 6 strains animals generally conform to the main biological characteristics of immunodeficient rats. However, certain lymphocyte subsets, blood physiological indexes, and organ coefficients are various in immunodeficient rats of the same strain from different manufacturers. The present study provides an important basis for establishment of criteria and application of standardized management for immunodeficient rats.

Key words: immunodeficient rats, hematology, lymphocyte subsets, organ coefficients

摘要： 目的 测试分析国内商品化免疫缺陷大鼠的血液淋巴细胞亚群百分率、免疫器官组织学、脏器系数及血液生 理指标等主要生物学特征。 方法 选取国内商品化销售 4 家公司的 3 类 6 个品系的免疫缺陷大鼠及相同遗传背景 的对照大鼠,检测并比较其血液生理学指标、血液淋巴细胞亚群、主要免疫器官的脏器系数及组织学特征。 结果 (1) T 淋巴细胞缺陷大鼠的胸腺系数及组织学特征与对照组比较差异有统计学意义( P < 0. 01) ;白细胞 (WBC) 、淋巴细胞( LYMPH)及其百分率和 T 细胞比率显著低于对照组(P<0. 01) ;中性粒细胞(NEUT)及其百分率 (NEUT%) 、B 细胞比率和自然杀伤(NK)细胞比率高于对照组( P<0. 01) ;不同厂家免疫缺陷大鼠在脾系数、WBC、 LYMPH 及百分率( LYMPH%) 、NEUT%及 B 细胞比率差异有统计学意义(P<0. 01) 。 (2) T、B 淋巴细胞联合缺陷大 鼠脾系数和胸腺系数及组织学特征与对照组差异有统计学意义(均 P<0. 01) ;WBC、LYMPH 及百分率、T 细胞比率 和 B 细胞比率均显著低于对照组(P<0. 01) ,单核细胞百分率( MONO%) 、NEUT 及其百分率、NK 细胞比率显著高 于对照组(P<0. 01) 。 (3) T、B、NK 细胞联合缺陷大鼠脾系数和胸腺系数均显著低于对照组( P< 0. 05,P< 0. 01) ; WBC、LYMPH 及其百分率( NEUT%) 、T 细胞和 B 细胞的比率均显著低于对照组( P< 0. 01) ;NEUT% 与 MONO% 数 值高于对照组(P<0. 01) ;不同公司 T、B、NK 细胞联合缺陷大鼠的 LYMPH%、NEUT%与 MONO%间均存在显著差异 (P<0. 01) 。 结论 6 个品系动物的免疫细胞数量和免疫器官组织学及血液学指标基本符合免疫缺陷大鼠的主要 生物学特征,但同一品系不同生产厂家来源的动物在某些淋巴细胞亚群、血液生理学指标及脏器系数上存在差异。 本研究为制定相关标准和实现标准化管理提供了重要依据。 

关键词: 免疫缺陷大鼠, 血液学, 淋巴细胞亚群, 脏器系数